Shape-Out is the dedicated analysis and visualization platform for real-time deformability cytometry (RT-DC) data.
It enables comprehensive post-processing of single-cell mechanical measurements, providing full access to event-level data and population statistics.
Key capabilities include:
Interactive gating and filtering across all recorded parameters
Visualization of deformation, cell size and fluorescence signals
Click-to-inspect linking between data points and corresponding cell images
Dataset comparison and multi-sample analysis
Calculation of elastic moduli
Export of statistics and plots for publication
Every measurement can be explored at single-cell resolution while maintaining robust population-level summaries.
Shape-Out is designed to ensure traceable and reproducible data evaluation.
All processing steps remain transparent and can be reviewed, adjusted and documented.
Users retain full control over gating strategies, parameter definitions and statistical evaluation.
The platform supports:
Parameter-based filtering and subpopulation analysis
Visualization of gated cell populations
Statistical comparison of datasets
Export of raw and processed data
Standard data formats for integration with external tools
By combining single-event traceability with structured statistical output, Shape-Out supports reproducible research workflows and multi-study comparisons.
Shape-Out is continuously developed to meet the evolving needs of RT-DC users.
The current publicly available version provides full data analysis functionality and supports installation on Windows and macOS systems.
It includes:
Interactive visualization
Multi-parameter gating
Elastic modulus calculation
Export in common data formats
Documentation and installation instructions are available via the download page.
A new major release of Shape-Out is currently in preparation.
The upcoming version will extend performance, usability and analysis capabilities while maintaining compatibility with RT-DC data formats.
Further details will be announced upon release.
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