RT-DC Data Analysis & Visualization Software

Shape-Out — Analysis Platform for Real-Time Deformability Cytometry

Built for Single-Cell Mechanical Data

Shape-Out is the dedicated analysis and visualization platform for real-time deformability cytometry (RT-DC) data.

It enables comprehensive post-processing of single-cell mechanical measurements, providing full access to event-level data and population statistics.

Key capabilities include:

  • Interactive gating and filtering across all recorded parameters

  • Visualization of deformation, cell size and fluorescence signals

  • Click-to-inspect linking between data points and corresponding cell images

  • Dataset comparison and multi-sample analysis

  • Calculation of elastic moduli

  • Export of statistics and plots for publication

Every measurement can be explored at single-cell resolution while maintaining robust population-level summaries.

Transparent and Reproducible Data Processing

Shape-Out is designed to ensure traceable and reproducible data evaluation.

All processing steps remain transparent and can be reviewed, adjusted and documented.
Users retain full control over gating strategies, parameter definitions and statistical evaluation.

The platform supports:

  • Parameter-based filtering and subpopulation analysis

  • Visualization of gated cell populations

  • Statistical comparison of datasets

  • Export of raw and processed data

  • Standard data formats for integration with external tools

By combining single-event traceability with structured statistical output, Shape-Out supports reproducible research workflows and multi-study comparisons.

Version Information

Shape-Out is continuously developed to meet the evolving needs of RT-DC users.

Current Release – Shape-Out 3

Shape-Out 3 is the latest version of our RT-DC data analysis software. It is available for Windows 11 and macOS Tahoe and supports datasets generated with AcCellerator systems.

The latest release, documentation and installation instructions are available on our download page.

Shape-Out is actively maintained and continuously improved. New features, performance enhancements and usability improvements are regularly incorporated based on user feedback and ongoing development.

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