Publications related to or based on
real-time deformability cytometry  


A comparison of microfluidic methods for high-throughput cell deformability measurements

Nature Methods 2020
 Urbanska M., Munoz H.E.,  Bagnall J.S., Otto O., Manalis S.R., Di Carlo D., Guck J.

High-throughput cell and spheroid mechanics in virtual fluidic channels

Nature  Communications 2020
Panhwar M.H., Czerwinski F., Dabbiru V.A.S., Komaragiri Y., Fregin B., Biedenweg D., Nestler P., Pires R.H., Otto O.

Quantifying single-platelet biomechanics: An outsider's guide to biophysical methods and recent advances

Res Pract Thromb Haemost  2020
Sachs L., Denker C., Greinacher A., Palankar R.

AIDeveloper: deep learning image classification in life science and beyond

Kräter M., Abuhattum S., Soteriou D., Jacobi A., Krüger T., Guck J., Herbig M.

Oncogenic Signaling Alters Cell Shape and Mechanics to Facilitate Cell Division under Confinement

Developmental Cell 2020 
Matthews HK., Ganguli S., Plak K., Taubenberger AV., Win Z., Williamson M., Piel M., Guck J., Baum B.

The mechanics of myeloid cells                                                                                                    

Biol. Cell 2020
Bashant KR., Toepfner N., Day CJ., Mehta NN., Kaplan MJ., Summers C., Guck J., Chilvers ER.


Cell Mechanics Based Computational Classification of Red Blood Cells Via Machine Intelligence Applied to Morpho-Rheological Markers

IEEE/ACM Trans Comput Biol Bioinform. 2019
Ge Y., Rosendahl P., Duran C., Toepfner N., Ciucci S., Guck J., Cannistraci C.V..

Morpho-Rheological Fingerprinting of Rod Photoreceptors Using Real-Time Deformability Cytometry

Cytometry A. 2019
Santos-Ferreira T., Herbig M., Otto O., Carido M., Karl MO., Michalakis S., Guck J., Ader M.

Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry

Methods Mol Biol. 2019
Jacobi A., Rosendahl P., Kräter M., Urbanska M., Herbig M., Guck J.                          

High-Throughput Microfluidic Characterization of Erythrocyte Shapes and Mechanical Variability                                            

Biophysical Journal 2019
Reichel F., Mauer J., Nawaz A.A., Gompper G., Guck J., Fedosov D.A.                          

The relationship between metastatic potential and in vitro mechanical properties of osteosarcoma cells

Molecular Biology of the Cell 2019
Holenstein C.N., Horvath A., Schär B., Schoenenberger A.D., Bollhalder M., Goedecke N., Bartalena G., Otto O., Herbig M., Guck J., Müller D.A., Snedeker J.G., Silvan U.

Cardiomyocyte mechanodynamics under conditions of actin remodelling

Philos Trans R Soc Lond B Biol Sci. 2019
Pires RH., Shree N., Manu E., Guzniczak E., Otto O.

High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry

Nature Communications 2019
Fregin B., Czerwinski F., Biedenweg D., Girardo S., Gross S., Aurich K., Otto O.

Real-time deformability cytometry reveals sequential contraction and expansion during neutrophil priming

Journal of Leukocyte Biology 2019
Bashant K.R., Vassallo A., Herold C., Berner R., Menschner L., Subburayalu J., Kaplan M.J., Summers C., Guck J., Chilvers E.R., Toepfner N.

Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis

Stem Cell Research & Therapy 2019
Sarem M., Otto O., Tanaka S., Shastri V.P.

Spheroid Culture of Mesenchymal Stromal Cells Results in Morphorheological Properties Appropriate for Improved Microcirculation

Advanced Science 2019
Tietze S., Kräter M., Jacobi A., Taubenberger A., Herbig M., Wehner R., Schmitz M., Otto O., List C., Kaya B., Wobus M., Bornhäuser M., Guck J.


Detection Of Human Disease Conditions By Single-Cell Morpho-Rheological Phenotyping Of Whole Blood

eLife. 2018
Töpfner N., Herold C., Otto O., Rosendahl P., Jacobi A., Kräter M., Stächele J., Menschner L., Herbig M., Ciuffreda L., Ranford-Cartwright L., Grzybek M., Coskun U., Reithuber E., Garriss G., Mellroth P., Henriques-Normark B., Tregay N., Suttorp M., Bornhäuser M., Chilvers E.R., Berner R., Guck J.

A comparison of methods to assess cell mechanical properties

Nature Methods 2018
Wu P.H., Aroush D.R., Asnacios A., Chen W.C., Dokukin M.E., Doss B.L., Durand-Smet P., Ekpenyong A., Guck J., Guz N.V., Janmey P.A., Lee J.S.H., Moore N.M., Ott A., Poh Y.C., Ros R., Sander M, Sokolov I, Staunton JR, Wang N, Whyte G, Wirtz D.

Standardized microgel beads as elastic cell mechanical probes

J Mater Chem B. 2018
Girardo S., Träber N., Wagner K., Cojoc G., Herold C., Goswami R., Schlüßler R., Abuhattum S., Taubenberger A., Reichel F., Mokbel D., Herbig M., Schürmann M., Müller P., Heida T., Jacobi A., Ulbricht E., Thiele J., Werner C., Guck J.

Biallelic CCM3 mutations cause a clonogenic survival advantage and endothelial cell stiffening

Journal of Cellular and Molecular Medicine 2018
Schwefel K., Spiegler S., Ameling S., Much C.D., Pilz R.A., Otto O., Völker U., Felbor U., Rath M.

Impact of poloxamer 188 (Pluronic F-68) additive on cell mechanical properties, quantification by real-time deformability cytometry

Biomicrofluidics 2018
Guzniczak E., Jimenez M., Irwin M., Otto O., Willoughby N., Bridle H.

Real-time fluorescence and deformability cytometry

Nature Methods 2018
Rosendahl P., Plak K., Jacobi A., Kraeter M., Toepfner N., Otto O., Herold C., Winzi M., Herbig M., Ge Y., Girardo S., Wagner K., Baum B., Guck J.

There Will Be Blood Tests

Cell 2018
Stacie E. Dodgson

Metabolic Profiling of Human Eosinophils

Front. Immunol. 2018
Porter L., Toepfner N., Bashant K.R., Guck J., Ashcroft M., Farahi N. and Chilvers E.R.

Alterations in Cell Mechanics by Actin Cytoskeletal Changes Correlate with Strain-Specific Rubella Virus Phenotypes for Cell Migration and Induction of Apoptosis

Cells 2018
Kräter M., Sapudom J., Bilz N.C., Pompe T., Guck J. and Claus C.

Statistics for real-time deformability cytometry: Clustering, dimensionality reduction,
and significance testing.

Biomicrofluidics 2018
Herbig M., Mietke A., Müller P., Otto O.

High-throughput single-cell mechanical phenotyping with real-time deformability cytometry

Methods in Cell Biology 2018
Urbanska M., Rosendahl P., Kräter M., Guck J.

Cancer cell ability to mechanically adjust to extracellular matrix stiffness correlates with their invasive potential

Molecular Biology of the Cell 2018
Wullkopf l., West AK. V., Leijnse N., Cox T. R., Madsen C. D., Oddershede L. B., Erler J.T.


Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells.

Flow Cytometry Protocols 2017
Herbig M., Kräter M., Plak K., Müller P., Guck J., Otto O.

Bone marrow niche-mimetics modulate HSPC function via integrin signaling.

Scientific Reports 2017
Kräter M., Jacobi A., Otto O., Tietze S., Müller K., Poitz DM., Palm S., Zinna VM., Biehain U., Wobus M., Chavakis T., Werner C., Guck J., Bornhäuser M.

Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage

Development 2017
Urbanska M., Winzi M., Neumann K., Abuhattum S., Rosendahl P.,  Müller P., Taubenberger A., Anastassiadis K., Guck J.

High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing

Scientific Reports 2017
Guzniczak E., Mohammad Zadeh M., Dempsey F., Jimenez M., Bock H., Whyte G., Willoughby N. & Bridle H.

Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion

BioRxiv. preprint. 2017
Surcel A., Schiffhauer E.S., Thomas D., Zhu Q., DiNapoli K., Herbig M., Otto O., Guck J., Jaffee E., Iglesias P., Anders R., Robinson D.

Initiation of acute graft-versus-host disease by angiogenesis

Blood. 2017
Riesner K, Shi Y, Jacobi A, Kraeter M, Kalupa M, McGearey A, Mertlitz S, Cordes S, Schrezenmeier JF, Mengwasser J, Westphal S, Perez-Hernandez D, Schmitt C, Dittmar G, Guck J, Penack O.

Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion.

PNAS. 2017
Koch M., Wright KE., Otto O., Herbig M., Salinas ND., Tolia NH., Satchwell TJ., Guck J., Brooks NJ., Baum J.

Toxicity and Immunogenicity in Murine Melanoma following Exposure to Physical Plasma-Derived Oxidants

Oxidative Medicine and Cellular Longevity. 2017
Bekeschus S., Rödder K., Fregin B., Otto O., Lippert M., Weltmann KD., Wende K., Schmidt A., Gandhirajan RK.

Numerical Simulation of Real-Time Deformability Cytometry To Extract Cell Mechanical Properties

ACS Biomater. Sci. Eng. 2017
Mokbel M., Mokbel D., Mietke A., Träber N., Girardo S., Otto O., Guck J., and Aland S.

Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells

Nature Communications. 2017
Tavares S., Vieira AF., Taubenberger AV., Araújo M., Martins NP., Brás-Pereira C., Polónia A., Herbig M., Barreto C., Otto O., Cardoso J., Pereira-Leal JB., Guck J., Paredes J., Janody F.

High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications.

Cytoskeleton. 2017
Golfier S., Rosendahl P., Mietke A., Herbig M., Guck J., Otto O.

Mapping of Deformation to Apparent Young's Modulus in Real-Time Deformability Cytometry 2017
Herold C.


V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling - a new option for HCC therapy

Oncotarget. 2016
Bartel K., Winzi M., Ulrich M., Koeberle A., Menche D., Werz O., Müller R., Guck J., Vollmar A.M, von Schwarzenberg K.

The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512

Mol Metab. 2016
Mziaut H, Mulligan B, Hoboth P, Otto O, Ivanova A, Herbig M, Schumann D, Hildebrandt T, Dehghany J, Sönmez A, Münster C, Meyer-Hermann M, Guck J, Kalaidzidis Y, Solimena M.

pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy

eLife 2016
M. C. Munder, D. Midtvedt, T. Franzmann, E. Nüske, O. Otto, M. Herbig, E. Ulbricht, P. Müller, A. Taubenberger, S. Maharana, L. Malinovska, D. Richter, J. Guck, V. Zaburdaev, S. Alberti. 

Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry

Integrative Biology 2016
M. Xavier, P. Rosendahl, M. Herbig, M. Kräter, D. Spencer, M. Bornhäuser, R. O. C. Oreffo, H. Morgan, J. Guck, O. Otto.


Real-time deformability cytometry as a label-free indicator of cell function

Conf Proc IEEE Eng Med Biol Soc. 2015
Otto O., Rosendahl P., Golfier S., Mietke A., Herbig M., Jacobi A., Topfner N., Herold C., Klaue D., Girardo S., Winzi M., Fischer-Friedrich E., Guck J.

Myosin II Activity Softens Cells in Suspension

Biophysical Journal 2015
C. J. Chan, A. E. Ekpenyong, S. Golfier, W. Li, K. J. Chalut, O. Otto, J. Elgeti, J. Guck, F. Lautenschläger

Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia

Oncotarget 2015
M. Wobus, M. Bornhäuser, J. Guck, O. Otto, A. Jacobi, M. Kräter, C. Ortlepp, G. Ehninger, Ch. Thiede, U. Oelschlägel

Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment

Biophysical Journal 2015
A. Mietke, O. Otto, S. Girardo, P. Rosendahl, A. Taubenberger, S. Golfier, E. Ulbricht, S. Aaland, J. Guck, E. Fischer-Friedrich

Cell Mechanics: Combining Speed with Precision

Biophysical Journal 2015
Wyss H.M.

Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

Nature Methods 2015
O. Otto, Ph. Rosendahl, A. Mietke, S. Golfier, Ch. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, J. Guck

Mechanics Meets Medicine

Science Translational Medicine 2013
Guck J., Chilvers E.R.

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