Publications related to or based on
real-time deformability cytometry  

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          2022 - Frontiers in Physiology

          Changes in Blood Cell Deformability in Chorea-Acanthocytosis and Effects of Treatment With Dasatinib or Lithium.

          Authors:
          Reichel F., Kräter M., Peikert K., Glaß H., Rosendahl P., Herbig M., Rivera Prieto A., Kihm A., Bosman G., Kaestner L., Hermann A., Guck J.

          2022 - Translational Psychiatry

          Depressive disorders are associated with increased peripheral blood cell deformability: a cross-sectional case-control study (Mood-Morph)

          Authors:
          Walther A., Mackens-Kiani A., Eder J., Herbig M., Herold C., Kirschbaum C., Guck J., Wittwer L.D., Beesdo-Baum K., Kräter, M.

          2022 - Communications Biology

          Ex vivo anticoagulants affect human blood platelet biomechanics with implications for high-throughput functional mechanophenotyping.

          Authors:
          Sachs L., Wesche J., Lenkeit L., Greinacher A., Bender M., Otto O., Palankar R.

          2022 - Scientific Reports

          Label-free imaging flow cytometry for analysis and sorting of enzymatically dissociated tissues.

          Authors:
          Herbig M., Tessmer K., Nötzel M., Nawaz A.A., Santos-Ferreira T., Borsch O., Gasparini S.J., Guck J., Ader M.

          2022 - Scientific Reports

          Machine learning assisted real‑time deformability cytometry of CD34+ cells allows to identify patients with myelodysplastic syndromes.

          Authors:
          Herbig M., Jacobi A., Wobus M., Weidner H., Mies A., Kräter M., Otto O., Thiede C., Weickert M., Götze K.S., Rauner M., Hofbauer L.C., Bornhäuser M., Guck J., Ader M., Platzbecker U., Balaian E.

          2021 - Blood

          HIF2α is a direct regulator of neutrophil motility

          Authors:
          Sormendi S., Deygas M., Sinha A., Bernard M., Krüger A., Kourtzelis I., Le Lay G., Sáez P.J., Gerlach M., Franke K., Meneses A., Kräter M., Palladini A., Guck J., Coskun Ü., Chavakis T., Vargas P., Wielockx B.

          2021 - Advanced Science

          AIDeveloper: Deep Learning Image Classification in Life Science and Beyond

          Authors:
          Kräter M., Abuhattum S., Soteriou D., Jacobi A., Krüger T., Guck J., Herbig M.

          2021 - Journal of Cachexia, Sarcopenia and Muscle

          Inhibition of the NLRP3/IL‐1β axis protects against sepsis‐induced cardiomyopathy

          Authors:
          Busch K., Kny M., Huang N., Klassert T.E., Stock M., Hahn A., Graeger S., Todiras M., Schmidt S., Chamling B., Willenbrock M., Groß S., Biedenweg D., Heuser A., Scheidereit C., Butter C., Felix S.B., Otto O., Luft F.C., Slevogt H., Fielitz J.

          2021 - Developmental Cell

          Mechanical Adaptability of Tumor Cells in Metastasis

          Authors:
          Gensbittel V, Kräter M, Harlepp S, Busnelli I, Guck J, Goetz JG.

          2021 - Biomaterials Science

          Mechanical properties of cell- and microgel bead-laden oxidized alginate-gelatin hydrogels

          Authors:
          Distler T., Kretzschmar L., Schneidereit D., Girardo S., Goswami R., Friedrich O., Detsch R., Guck J., Boccaccini A.R., Budday S.

          2021 - Biophysical Journal

          Physical phenotype of blood cells is altered in COVID-19

          Authors:
          Kubánková M., Hohberger B., Hoffmanns J., Fürst J., Herrmann M., Guck J., Kräter M.

          2021 - Annals of the Rheumatic Diseases

          Proteomic, biomechanical and functional analyses define neutrophil heterogeneity in systemic lupus erythematosus.

          Authors:
          Bashant KR, Aponte AM, Randazzo D, Rezvan Sangsari P, Wood AJ, Bibby JA, West EE, Vassallo A, Manna ZG, Playford MP, Jordan N, Hasni S, Gucek M, Kemper C, Conway Morris A, Morgan NY, Toepfner N, Guck J, Mehta NN, Chilvers ER, Summers C, Kaplan MJ.

          2021 - Investigative Radiology

          Real-Time Deformability Cytometry Detects Leukocyte Stiffening After Gadolinium-Based Contrast Agent Exposure

          Authors:
          Jacobi A., Ariza de Schellenberger A., Uca Y.O., Herbig M., Guck J., Sack I.

          2020 - Nature Methods

          A comparison of microfluidic methods for high-throughput cell deformability measurements

          Authors:
          Urbanska M., Munoz H.E.,  Bagnall J.S., Otto O., Manalis S.R., Di Carlo D., Guck J.

          2020 - Materials Today Bio

          Cancer-associated fibroblasts of the prostate promote a compliant and more invasive phenotype in benign prostate epithelial cells

          Authors:
          Jaeschke A, Jacobi A, Lawrence MG, Risbridger GP, Frydenberg M, Williams ED, Vela I, Hutmacher DW, Bray LJ, Taubenberger A

          2020 - Haematologica

          Coactosin-like 1 integrates signaling critical for shear-dependent thrombus formation in mouse platelets

          Authors:
          Scheller I, Stritt S, Beck S, Peng B, Pleines I, Heinze KG, Braun A, Otto O, Ahrends R, Sickmann A, Bender M, Nieswandt B.

          2020 - Lab on a Chip

          Deformability-induced lift force in spiral microchannels for cell separation.

          Authors:
          Guzniczak E, Otto O, Whyte G, Willoughby N, Jimenez M, Bridle H.

          2020 - Nature  Communications

          High-throughput cell and spheroid mechanics in virtual fluidic channels

          Authors:
          Panhwar M.H., Czerwinski F., Dabbiru V.A.S., Komaragiri Y., Fregin B., Biedenweg D., Nestler P., Pires R.H., Otto O.

          2020 - Nature Methods

          Intelligent image-based deformation-assisted cell sorting with molecular specificity

          Authors:
          Nawaz AA, Urbanska M, Herbig M, Nötzel M, Kräter M, Rosendahl P, Herold C, Toepfner N, Kubánková M, Goswami R, Abuhattum S, Reichel F, Müller P, Taubenberger A, Girardo S, Jacobi A, Guck J.

          2020 - Lab on a Chip

          Label-free on chip quality assessment of cellular blood products using real-time deformability cytometry

          Authors:
          Aurich K., Fregin B., Palankar R., Wesche J., Hartwich O., Biedenweg D., Nguyen TH., Greinacher A., Otto O.

          2020 - Frontiers in Immunology

          Maturation of Monocyte-Derived DCs Leads to Increased Cellular Stiffness, Higher Membrane Fluidity, and Changed Lipid Composition.

          Authors:
          Löhr J.J., Alex N., Amon L., Kräter M., Kubánková M., Sezgin E., Lehmann C.H.K., Heger L., Heidkamp G.F., Smith A.-S., Zaburdaev V., Böckmann R.A., Levental I., Dustin M.L., Eggeling C., Guck J., Dudziak D.

          2020 - Polymers

          Microfluidic Fabrication of Click Chemistry-Mediated Hyaluronic Acid Microgels: A Bottom-Up Material Guide to Tailor a Microgel's Physicochemical and Mechanical Properties

          Authors:
          Heida T, Otto O, Biedenweg D, Hauck N, Thiele J.

          2020 - Developmental Cell

          Oncogenic Signaling Alters Cell Shape and Mechanics to Facilitate Cell Division under Confinement

          Authors:
          Matthews HK., Ganguli S., Plak K., Taubenberger AV., Win Z., Williamson M., Piel M., Guck J., Baum B.

          2020 - Biotechnoly and Bioengineering

          Purifying stem cell-derived red blood cells: a high-throughput label-free downstream processing strategy based on microfluidic spiral inertial separation and membrane filtration.

          Authors:
          Guzniczak E, Otto O, Whyte G, Chandra T, Robertson NA, Willoughby N, Jimenez M, Bridle H.

          2020 - Research and Practice in Thrombosis and Haemostasis

          Quantifying single-platelet biomechanics: An outsider's guide to biophysical methods and recent advances

          Authors:
          Sachs L., Denker C., Greinacher A., Palankar R.

          2020 - Hamostaseologie

          Role of Platelet Cytoskeleton in Platelet Biomechanics: Current and Emerging Methodologies and Their Potential Relevance for the Investigation of Inherited Platelet Disorders

          Authors:
          Zaninetti C, Sachs L, Palankar R.

          2020 - Biology of the Cell

          The mechanics of myeloid cells                                                                                                    

          Authors:
          Bashant KR., Toepfner N., Day CJ., Mehta NN., Kaplan MJ., Summers C., Guck J., Chilvers ER.

          2020 - Journal of Polymer Science

          Tunable polymer microgel particles and their study using microscopy and real‐time deformability cytometry

          Authors:
          Sinjari S, Freitag JS, Herold C, Otto O, Smith DM, Stöver HDH

          2019 - Methods in Molecular Biology

          Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry

          Authors:
          Jacobi A., Rosendahl P., Kräter M., Urbanska M., Herbig M., Guck J.

          2019 - Journal of Cellular and Molecular Medicine

          Biallelic CCM3 mutations cause a clonogenic survival advantage and endothelial cell stiffening

          Authors:
          Schwefel K., Spiegler S., Ameling S., Much C.D., Pilz R.A., Otto O., Völker U., Felbor U., Rath M.

          2019 - Philosophical Transactions of the Royal Society B: Biological Sciences

          Cardiomyocyte mechanodynamics under conditions of actin remodelling

          Authors:
          Pires RH., Shree N., Manu E., Guzniczak E., Otto O.

          2019 - IEEE/ACM Transactions on Computational Biology and Bioinformatics

          Cell Mechanics Based Computational Classification of Red Blood Cells Via Machine Intelligence Applied to Morpho-Rheological Markers

          Authors:
          Ge Y., Rosendahl P., Duran C., Toepfner N., Ciucci S., Guck J., Cannistraci C.V..

          2019 - Stem Cell Research & Therapy

          Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis

          Authors:
          Sarem M., Otto O., Tanaka S., Shastri V.P.

          2019 - Annals of Hematology

          Effects of rigosertib on the osteo-hematopoietic niche in myelodysplastic syndromes

          Authors:
          Balaian E., Weidner H., Wobus M., Baschant U., Jacobi A., Mies A., Bornhäuser M., Guck J., Hofbauer L.C., Rauner M., Platzbecker U.

          2019 - Biophysical Journal

          High-Throughput Microfluidic Characterization of Erythrocyte Shapes and Mechanical Variability                                            

          Authors:
          Reichel F., Mauer J., Nawaz A.A., Gompper G., Guck J., Fedosov D.A.

          2019 - Nature Communications

          High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry

          Authors:
          Fregin B., Czerwinski F., Biedenweg D., Girardo S., Gross S., Aurich K., Otto O.

          2019 - Cytometry Part A

          Morpho-Rheological Fingerprinting of Rod Photoreceptors Using Real-Time Deformability Cytometry

          Authors:
          Santos-Ferreira T., Herbig M., Otto O., Carido M., Karl MO., Michalakis S., Guck J., Ader M.

          2019 - Journal of Leukocyte Biology

          Real-time deformability cytometry reveals sequential contraction and expansion during neutrophil priming

          Authors:
          Bashant K.R., Vassallo A., Herold C., Berner R., Menschner L., Subburayalu J., Kaplan M.J., Summers C., Guck J., Chilvers E.R., Toepfner N.

          2019 - Advanced Science

          Spheroid Culture of Mesenchymal Stromal Cells Results in Morphorheological Properties Appropriate for Improved Microcirculation

          Authors:
          Tietze S., Kräter M., Jacobi A., Taubenberger A., Herbig M., Wehner R., Schmitz M., Otto O., List C., Kaya B., Wobus M., Bornhäuser M., Guck J.

          2019 - Cancer Research

          Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14

          Authors:
          Surcel A., Schiffhauer E.S., Thomas D.G., Zhu Q., DiNapoli K.T., Herbig M., Otto O., West-Foyle H., Jacobi A., Kräter M., Plak K., Guck J., Jaffee E.M., Iglesias P.A., Anders R.A., Robinson D.N.

          2019 - Molecular Biology of the Cell

          The relationship between metastatic potential and in vitro mechanical properties of osteosarcoma cells

          Authors:
          Holenstein C.N., Horvath A., Schär B., Schoenenberger A.D., Bollhalder M., Goedecke N., Bartalena G., Otto O., Herbig M., Guck J., Müller D.A., Snedeker J.G., Silvan U.

          2018 - Cells

          Alterations in Cell Mechanics by Actin Cytoskeletal Changes Correlate with Strain-Specific Rubella Virus Phenotypes for Cell Migration and Induction of Apoptosis

          Authors:
          Kräter M., Sapudom J., Bilz N.C., Pompe T., Guck J., Claus C.

          2018 - Molecular Biology of the Cell

          Cancer cell ability to mechanically adjust to extracellular matrix stiffness correlates with their invasive potential

          Authors:
          Wullkopf l., West AK. V., Leijnse N., Cox T. R., Madsen C. D., Oddershede L. B., Erler J.T.

          2018 - eLife

          Detection Of Human Disease Conditions By Single-Cell Morpho-Rheological Phenotyping Of Whole Blood

          Authors:
          Töpfner N., Herold C., Otto O., Rosendahl P., Jacobi A., Kräter M., Stächele J., Menschner L., Herbig M., Ciuffreda L., Ranford-Cartwright L., Grzybek M., Coskun U., Reithuber E., Garriss G., Mellroth P., Henriques-Normark B., Tregay N., Suttorp M., Bornhäuser M., Chilvers E.R., Berner R., Guck J.

          2018 - Methods in Cell Biology

          High-throughput single-cell mechanical phenotyping with real-time deformability cytometry

          Authors:
          Urbanska M., Rosendahl P., Kräter M., Guck J.

          2018 - Biomicrofluidics

          Impact of poloxamer 188 (Pluronic F-68) additive on cell mechanical properties, quantification by real-time deformability cytometry

          Authors:
          Guzniczak E., Jimenez M., Irwin M., Otto O., Willoughby N., Bridle H.

          2018 - Frontiers in Immunology

          Metabolic Profiling of Human Eosinophils

          Authors:
          Porter L., Toepfner N., Bashant K.R., Guck J., Ashcroft M., Farahi N., Chilvers E.R.

          2018 - Flow Cytometry Protocols

          Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells.

          Authors:
          Herbig M., Kräter M., Plak K., Müller P., Guck J., Otto O.

          2018 - Nature Methods

          Real-time fluorescence and deformability cytometry

          Authors:
          Rosendahl P., Plak K., Jacobi A., Kraeter M., Toepfner N., Otto O., Herold C., Winzi M., Herbig M., Ge Y., Girardo S., Wagner K., Baum B., Guck J.

          2018 - Journal of Materials Chemistry B

          Standardized microgel beads as elastic cell mechanical probes

          Authors:
          Girardo S., Träber N., Wagner K., Cojoc G., Herold C., Goswami R., Schlüßler R., Abuhattum S., Taubenberger A., Reichel F., Mokbel D., Herbig M., Schürmann M., Müller P., Heida T., Jacobi A., Ulbricht E., Thiele J., Werner C., Guck J.

          2018 - Biomicrofluidics

          Statistics for real-time deformability cytometry: Clustering, dimensionality reduction, and significance testing.

          Authors:
          Herbig M., Mietke A., Müller P., Otto O.

          2018 - Cell

          There Will Be Blood Tests

          Authors:
          Dodgson S.E.

          2017 - Nature Communications

          Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells

          Authors:
          Tavares S., Vieira AF., Taubenberger AV., Araújo M., Martins NP., Brás-Pereira C., Polónia A., Herbig M., Barreto C., Otto O., Cardoso J., Pereira-Leal JB., Guck J., Paredes J., Janody F.

          2017 - Scientific Reports

          Bone marrow niche-mimetics modulate HSPC function via integrin signaling.

          Authors:
          Kräter M., Jacobi A., Otto O., Tietze S., Müller K., Poitz DM., Palm S., Zinna VM., Biehain U., Wobus M., Chavakis T., Werner C., Guck J., Bornhäuser M.

          2017 - Scientific Reports

          High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing

          Authors:
          Guzniczak E., Mohammad Zadeh M., Dempsey F., Jimenez M., Bock H., Whyte G., Willoughby N. & Bridle H.

          2017 - Cytoskeleton

          High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications.

          Authors:
          Golfier S., Rosendahl P., Mietke A., Herbig M., Guck J., Otto O.

          2017 - Blood

          Initiation of acute graft-versus-host disease by angiogenesis

          Authors:
          Riesner K, Shi Y, Jacobi A, Kraeter M, Kalupa M, McGearey A, Mertlitz S, Cordes S, Schrezenmeier JF, Mengwasser J, Westphal S, Perez-Hernandez D, Schmitt C, Dittmar G, Guck J, Penack O.

          2017 - arXiv.org

          Mapping of Deformation to Apparent Young's Modulus in Real-Time Deformability Cytometry

          Authors:
          Herold C.

          2017 - ACS Biomaterials Science & Engineering

          Numerical Simulation of Real-Time Deformability Cytometry To Extract Cell Mechanical Properties

          Authors:
          Mokbel M., Mokbel D., Mietke A., Träber N., Girardo S., Otto O., Guck J., and Aland S.

          2017 - Proceedings of the National Academy of Sciences

          Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion.

          Authors:
          Koch M., Wright KE., Otto O., Herbig M., Salinas ND., Tolia NH., Satchwell TJ., Guck J., Brooks NJ., Baum J.

          2017 - Development

          Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage

          Authors:
          Urbanska M., Winzi M., Neumann K., Abuhattum S., Rosendahl P.,  Müller P., Taubenberger A., Anastassiadis K., Guck J.

          2017 - Oxidative Medicine and Cellular Longevity

          Toxicity and Immunogenicity in Murine Melanoma following Exposure to Physical Plasma-Derived Oxidants

          Authors:
          Bekeschus S., Rödder K., Fregin B., Otto O., Lippert M., Weltmann KD., Wende K., Schmidt A., Gandhirajan RK.

          2017 - Oncotarget

          V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling - a new option for HCC therapy

          Authors:
          Bartel K., Winzi M., Ulrich M., Koeberle A., Menche D., Werz O., Müller R., Guck J., Vollmar A.M, von Schwarzenberg K.

          2016 - eLife

          A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy

          Authors:
          M. C. Munder, D. Midtvedt, T. Franzmann, E. Nüske, O. Otto, M. Herbig, E. Ulbricht, P. Müller, A. Taubenberger, S. Maharana, L. Malinovska, D. Richter, J. Guck, V. Zaburdaev, S. Alberti. 

          2016 - Integrative Biology

          Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry

          Authors:
          M. Xavier, P. Rosendahl, M. Herbig, M. Kräter, D. Spencer, M. Bornhäuser, R. O. C. Oreffo, H. Morgan, J. Guck, O. Otto.

          2016 - Molecular Metabolism

          The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512

          Authors:
          Mziaut H, Mulligan B, Hoboth P, Otto O, Ivanova A, Herbig M, Schumann D, Hildebrandt T, Dehghany J, Sönmez A, Münster C, Meyer-Hermann M, Guck J, Kalaidzidis Y, Solimena M.

          2015 - Oncotarget

          Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia

          Authors:
          M. Wobus, M. Bornhäuser, J. Guck, O. Otto, A. Jacobi, M. Kräter, C. Ortlepp, G. Ehninger, Ch. Thiede, U. Oelschlägel

          2015 - Biophysical Journal

          Cell Mechanics: Combining Speed with Precision

          Authors:
          Wyss H.M.

          2015 - Biophysical Journal

          Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment

          Authors:
          A. Mietke, O. Otto, S. Girardo, P. Rosendahl, A. Taubenberger, S. Golfier, E. Ulbricht, S. Aaland, J. Guck, E. Fischer-Friedrich

          2015 - Biophysical Journal

          Myosin II Activity Softens Cells in Suspension

          Authors:
          C. J. Chan, A. E. Ekpenyong, S. Golfier, W. Li, K. J. Chalut, O. Otto, J. Elgeti, J. Guck, F. Lautenschläger

          2015 - Nature Methods

          Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

          Authors:
          O. Otto, Ph. Rosendahl, A. Mietke, S. Golfier, Ch. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, J. Guck

          2013 - Science Translational Medicine

          Mechanics Meets Medicine

          Authors:
          Guck J., Chilvers E.R.

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