Publications related to or based on
real-time deformability cytometry  

2020

AIDeveloper: deep learning image classification in life science and beyond

BioRxiv
Kräter M., Abuhattum S., Soteriou D., Jacobi A., Krüger T., Guck J., Herbig M.

Oncogenic Signaling Alters Cell Shape and Mechanics to Facilitate Cell Division under Confinement

Dev Cell. 2020 
Matthews HK., Ganguli S., Plak K., Taubenberger AV., Win Z., Williamson M., Piel M., Guck J., Baum B.

Biol. Cell 2020
Bashant KR., Toepfner N., Day CJ., Mehta NN., Kaplan MJ., Summers C., Guck J., Chilvers ER.

2019

Morpho-Rheological Fingerprinting of Rod Photoreceptors Using Real-Time Deformability Cytometry

Cytometry A. 2019
Santos-Ferreira T., Herbig M., Otto O., Carido M., Karl MO., Michalakis S., Guck J., Ader M.

The relationship between metastatic potential and in vitro mechanical properties of osteosarcoma cells

Molecular Biology of the Cell 2019
Holenstein C.N., Horvath A., Schär B., Schoenenberger A.D., Bollhalder M., Goedecke N., Bartalena G., Otto O., Herbig M., Guck J., Müller D.A., Snedeker J.G., Silvan U.

Cardiomyocyte mechanodynamics under conditions of actin remodelling

Philos Trans R Soc Lond B Biol Sci. 2019
Pires RH., Shree N., Manu E., Guzniczak E., Otto O.


High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry

Nature Communications 2019
Fregin B., Czerwinski F., Biedenweg D., Girardo S., Gross S., Aurich K., Otto O.

Real-time deformability cytometry reveals sequential contraction and expansion during neutrophil priming

Journal of Leukocyte Biology 2019
Bashant K.R., Vassallo A., Herold C., Berner R., Menschner L., Subburayalu J., Kaplan M.J., Summers C., Guck J., Chilvers E.R., Toepfner N.

Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis

Stem Cell Research & Therapy 2019
Sarem M., Otto O., Tanaka S., Shastri V.P.


Spheroid Culture of Mesenchymal Stromal Cells Results in Morphorheological Properties Appropriate for Improved Microcirculation

Advanced Science 2019
Tietze S., Kräter M., Jacobi A., Taubenberger A., Herbig M., Wehner R., Schmitz M., Otto O., List C., Kaya B., Wobus M., Bornhäuser M., Guck J.

2018

Detection Of Human Disease Conditions By Single-Cell Morpho-Rheological Phenotyping Of Whole Blood

eLife. 2018
Töpfner N., Herold C., Otto O., Rosendahl P., Jacobi A., Kräter M., Stächele J., Menschner L., Herbig M., Ciuffreda L., Ranford-Cartwright L., Grzybek M., Coskun U., Reithuber E., Garriss G., Mellroth P., Henriques-Normark B., Tregay N., Suttorp M., Bornhäuser M., Chilvers E.R., Berner R., Guck J.

Biallelic CCM3 mutations cause a clonogenic survival advantage and endothelial cell stiffening

Journal of Cellular and Molecular Medicine 2018
Schwefel K., Spiegler S., Ameling S., Much C.D., Pilz R.A., Otto O., Völker U., Felbor U., Rath M.

Impact of poloxamer 188 (Pluronic F-68) additive on cell mechanical properties, quantification by real-time deformability cytometry

Biomicrofluidics 2018
Guzniczak E., Jimenez M., Irwin M., Otto O., Willoughby N., Bridle H.

Real-time fluorescence and deformability cytometry


Nature Methods 2018
Rosendahl P., Plak K., Jacobi A., Kraeter M., Toepfner N., Otto O., Herold C., Winzi M., Herbig M., Ge Y., Girardo S., Wagner K., Baum B., Guck J.

There Will Be Blood Tests


Cell 2018
Stacie E. Dodgson

Metabolic Profiling of Human Eosinophils


Front. Immunol. 2018
Porter L., Toepfner N., Bashant K.R., Guck J., Ashcroft M., Farahi N. and Chilvers E.R.

Alterations in Cell Mechanics by Actin Cytoskeletal Changes Correlate with Strain-Specific Rubella Virus Phenotypes for Cell Migration and Induction of Apoptosis

Cells 2018
Kräter M., Sapudom J., Bilz N.C., Pompe T., Guck J. and Claus C.

Statistics for real-time deformability cytometry: Clustering, dimensionality reduction,
and significance testing.

Biomicrofluidics 2018
Herbig M., Mietke A., Müller P., Otto O.

High-throughput single-cell mechanical phenotyping with real-time deformability cytometry

Methods in Cell Biology 2018
Urbanska M., Rosendahl P., Kräter M., Guck J.

Cancer cell ability to mechanically adjust to extracellular matrix stiffness correlates with their invasive potential

Molecular Biology of the Cell 2018
Wullkopf l., West AK. V., Leijnse N., Cox T. R., Madsen C. D., Oddershede L. B., Erler J.T.

2017

Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells.

Flow Cytometry Protocols 2017
Herbig M., Kräter M., Plak K., Müller P., Guck J., Otto O.


Bone marrow niche-mimetics modulate HSPC function via integrin signaling.

Scientific Reports 2017
Kräter M., Jacobi A., Otto O., Tietze S., Müller K., Poitz DM., Palm S., Zinna VM., Biehain U., Wobus M., Chavakis T., Werner C., Guck J., Bornhäuser M.

Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage

Development 2017
Marta Urbanska, Maria Winzi, Katrin Neumann, Shada Abuhattum, Philipp Rosendahl, Paul Müller, Anna Taubenberger, Konstantinos Anastassiadis, Jochen Guck

High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing

Scientific Reports 2017
Guzniczak E., Mohammad Zadeh M., Dempsey F., Jimenez M., Bock H., Whyte G., Willoughby N. & Bridle H.

Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion

BioRxiv. preprint. 2017
Surcel A., Schiffhauer E.S., Thomas D., Zhu Q., DiNapoli K., Herbig M., Otto O., Guck J., Jaffee E., Iglesias P., Anders R., Robinson D.

Initiation of acute graft-versus-host disease by angiogenesis

Blood. 2017
Riesner K, Shi Y, Jacobi A, Kraeter M, Kalupa M, McGearey A, Mertlitz S, Cordes S, Schrezenmeier JF, Mengwasser J, Westphal S, Perez-Hernandez D, Schmitt C, Dittmar G, Guck J, Penack O.

Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion.

PNAS. 2017
Koch M., Wright KE., Otto O., Herbig M., Salinas ND., Tolia NH., Satchwell TJ., Guck J., Brooks NJ., Baum J.

Toxicity and Immunogenicity in Murine Melanoma following Exposure to Physical Plasma-Derived Oxidants

Oxidative Medicine and Cellular Longevity. 2017
Bekeschus S., Rödder K., Fregin B., Otto O., Lippert M., Weltmann KD., Wende K., Schmidt A., Gandhirajan RK.

Numerical Simulation of Real-Time Deformability Cytometry To Extract Cell Mechanical Properties

ACS Biomater. Sci. Eng. 2017
Mokbel M., Mokbel D., Mietke A., Träber N., Girardo S., Otto O., Guck J., and Aland S.

Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells

Nature Communications. 2017
Tavares S., Vieira AF., Taubenberger AV., Araújo M., Martins NP., Brás-Pereira C., Polónia A., Herbig M., Barreto C., Otto O., Cardoso J., Pereira-Leal JB., Guck J., Paredes J., Janody F.

High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications.

Cytoskeleton. 2017
Golfier S., Rosendahl P., Mietke A., Herbig M., Guck J., Otto O.


Mapping of Deformation to Apparent Young's Modulus in Real-Time Deformability Cytometry

arXiv.org. 2017
Herold C.


2016

V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling - a new option for HCC therapy

Oncotarget. 2016
Bartel K, Winzi M, Ulrich M, Koeberle A, Menche D, Werz O, Müller R, Guck J, Vollmar AM, von Schwarzenberg K.

The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512

Mol Metab. 2016
Mziaut H, Mulligan B, Hoboth P, Otto O, Ivanova A, Herbig M, Schumann D, Hildebrandt T, Dehghany J, Sönmez A, Münster C, Meyer-Hermann M, Guck J, Kalaidzidis Y, Solimena M.

pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy

eLife 2016
M. C. Munder, D. Midtvedt, T. Franzmann, E. Nüske, O. Otto, M. Herbig, E. Ulbricht, P. Müller, A. Taubenberger, S. Maharana, L. Malinovska, D. Richter, J. Guck, V. Zaburdaev and S. Alberti. A

Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry

Integrative Biology 2016
M. Xavier, P. Rosendahl, M. Herbig, M. Kräter, D. Spencer, M. Bornhäuser, R. O. C. Oreffo, H. Morgan, J. Guck and O. Otto.

2015

Myosin II Activity Softens Cells in Suspension


Biophysical Journal 2015
C. J. Chan, A. E. Ekpenyong, S. Golfier, W. Li, K. J. Chalut, O. Otto, J. Elgeti, J. Guck and F. Lautenschläger

Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia

Oncotarget 2015
M. Wobus, M. Bornhäuser, J. Guck, O. Otto, A. Jacobi, M. Kräter, C. Ortlepp, G. Ehninger, Ch. Thiede, and U. Oelschlägel

Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment

Biophysical Journal 2015
A. Mietke, O. Otto, S. Girardo, P. Rosendahl, A. Taubenberger, S. Golfier, E. Ulbricht, S. Aaland, J. Guck and E. Fischer-Friedrich

Cell Mechanics: Combining Speed with Precision

Biophysical Journal 2015
Hans M. Wyss


Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

Nature Methods 2015
O. Otto, Ph. Rosendahl, A. Mietke, S. Golfier, Ch. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, and J. Guck

Mechanics Meets Medicine

Science Translational Medicine 2013
Jochen Guck and Edwin R. Chilvers


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