Publications related to or based on
real-time deformability cytometry
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2022 - Frontiers in Physiology
Changes in Blood Cell Deformability in Chorea-Acanthocytosis and Effects of Treatment With Dasatinib or Lithium.
Authors:Reichel F., Kräter M., Peikert K., Glaß H., Rosendahl P., Herbig M., Rivera Prieto A., Kihm A., Bosman G., Kaestner L., Hermann A., Guck J.
2022 - Translational Psychiatry
Depressive disorders are associated with increased peripheral blood cell deformability: a cross-sectional case-control study (Mood-Morph)
Authors:Walther A., Mackens-Kiani A., Eder J., Herbig M., Herold C., Kirschbaum C., Guck J., Wittwer L.D., Beesdo-Baum K., Kräter, M.
2022 - Communications Biology
Ex vivo anticoagulants affect human blood platelet biomechanics with implications for high-throughput functional mechanophenotyping.
Authors:Sachs L., Wesche J., Lenkeit L., Greinacher A., Bender M., Otto O., Palankar R.
2022 - Scientific Reports
Label-free imaging flow cytometry for analysis and sorting of enzymatically dissociated tissues.
Authors:Herbig M., Tessmer K., Nötzel M., Nawaz A.A., Santos-Ferreira T., Borsch O., Gasparini S.J., Guck J., Ader M.
2022 - Scientific Reports
Machine learning assisted real‑time deformability cytometry of CD34+ cells allows to identify patients with myelodysplastic syndromes.
Authors:Herbig M., Jacobi A., Wobus M., Weidner H., Mies A., Kräter M., Otto O., Thiede C., Weickert M., Götze K.S., Rauner M., Hofbauer L.C., Bornhäuser M., Guck J., Ader M., Platzbecker U., Balaian E.
2021 - Blood
HIF2α is a direct regulator of neutrophil motility
Authors:Sormendi S., Deygas M., Sinha A., Bernard M., Krüger A., Kourtzelis I., Le Lay G., Sáez P.J., Gerlach M., Franke K., Meneses A., Kräter M., Palladini A., Guck J., Coskun Ü., Chavakis T., Vargas P., Wielockx B.
2021 - Advanced Science
AIDeveloper: Deep Learning Image Classification in Life Science and Beyond
Authors:Kräter M., Abuhattum S., Soteriou D., Jacobi A., Krüger T., Guck J., Herbig M.
2021 - Journal of Cachexia, Sarcopenia and Muscle
Inhibition of the NLRP3/IL‐1β axis protects against sepsis‐induced cardiomyopathy
Authors:Busch K., Kny M., Huang N., Klassert T.E., Stock M., Hahn A., Graeger S., Todiras M., Schmidt S., Chamling B., Willenbrock M., Groß S., Biedenweg D., Heuser A., Scheidereit C., Butter C., Felix S.B., Otto O., Luft F.C., Slevogt H., Fielitz J.
2021 - Developmental Cell
Mechanical Adaptability of Tumor Cells in Metastasis
Authors:Gensbittel V, Kräter M, Harlepp S, Busnelli I, Guck J, Goetz JG.
2021 - Biomaterials Science
Mechanical properties of cell- and microgel bead-laden oxidized alginate-gelatin hydrogels
Authors:Distler T., Kretzschmar L., Schneidereit D., Girardo S., Goswami R., Friedrich O., Detsch R., Guck J., Boccaccini A.R., Budday S.
2021 - Biophysical Journal
Physical phenotype of blood cells is altered in COVID-19
Authors:Kubánková M., Hohberger B., Hoffmanns J., Fürst J., Herrmann M., Guck J., Kräter M.
2021 - Annals of the Rheumatic Diseases
Proteomic, biomechanical and functional analyses define neutrophil heterogeneity in systemic lupus erythematosus.
Authors:Bashant KR, Aponte AM, Randazzo D, Rezvan Sangsari P, Wood AJ, Bibby JA, West EE, Vassallo A, Manna ZG, Playford MP, Jordan N, Hasni S, Gucek M, Kemper C, Conway Morris A, Morgan NY, Toepfner N, Guck J, Mehta NN, Chilvers ER, Summers C, Kaplan MJ.
2021 - Investigative Radiology
Real-Time Deformability Cytometry Detects Leukocyte Stiffening After Gadolinium-Based Contrast Agent Exposure
Authors:Jacobi A., Ariza de Schellenberger A., Uca Y.O., Herbig M., Guck J., Sack I.
2020 - Nature Methods
A comparison of microfluidic methods for high-throughput cell deformability measurements
Authors:Urbanska M., Munoz H.E., Bagnall J.S., Otto O., Manalis S.R., Di Carlo D., Guck J.
2020 - Materials Today Bio
Cancer-associated fibroblasts of the prostate promote a compliant and more invasive phenotype in benign prostate epithelial cells
Authors:Jaeschke A, Jacobi A, Lawrence MG, Risbridger GP, Frydenberg M, Williams ED, Vela I, Hutmacher DW, Bray LJ, Taubenberger A
2020 - Haematologica
Coactosin-like 1 integrates signaling critical for shear-dependent thrombus formation in mouse platelets
Authors:Scheller I, Stritt S, Beck S, Peng B, Pleines I, Heinze KG, Braun A, Otto O, Ahrends R, Sickmann A, Bender M, Nieswandt B.
2020 - Lab on a Chip
Deformability-induced lift force in spiral microchannels for cell separation.
Authors:Guzniczak E, Otto O, Whyte G, Willoughby N, Jimenez M, Bridle H.
2020 - Nature Communications
High-throughput cell and spheroid mechanics in virtual fluidic channels
Authors:Panhwar M.H., Czerwinski F., Dabbiru V.A.S., Komaragiri Y., Fregin B., Biedenweg D., Nestler P., Pires R.H., Otto O.
2020 - Nature Methods
Intelligent image-based deformation-assisted cell sorting with molecular specificity
Authors:Nawaz AA, Urbanska M, Herbig M, Nötzel M, Kräter M, Rosendahl P, Herold C, Toepfner N, Kubánková M, Goswami R, Abuhattum S, Reichel F, Müller P, Taubenberger A, Girardo S, Jacobi A, Guck J.
2020 - Lab on a Chip
Label-free on chip quality assessment of cellular blood products using real-time deformability cytometry
Authors:Aurich K., Fregin B., Palankar R., Wesche J., Hartwich O., Biedenweg D., Nguyen TH., Greinacher A., Otto O.
2020 - Frontiers in Immunology
Maturation of Monocyte-Derived DCs Leads to Increased Cellular Stiffness, Higher Membrane Fluidity, and Changed Lipid Composition.
Authors:Löhr J.J., Alex N., Amon L., Kräter M., Kubánková M., Sezgin E., Lehmann C.H.K., Heger L., Heidkamp G.F., Smith A.-S., Zaburdaev V., Böckmann R.A., Levental I., Dustin M.L., Eggeling C., Guck J., Dudziak D.
2020 - Polymers
Microfluidic Fabrication of Click Chemistry-Mediated Hyaluronic Acid Microgels: A Bottom-Up Material Guide to Tailor a Microgel's Physicochemical and Mechanical Properties
Authors:Heida T, Otto O, Biedenweg D, Hauck N, Thiele J.
2020 - Developmental Cell
Oncogenic Signaling Alters Cell Shape and Mechanics to Facilitate Cell Division under Confinement
Authors:Matthews HK., Ganguli S., Plak K., Taubenberger AV., Win Z., Williamson M., Piel M., Guck J., Baum B.
2020 - Biotechnoly and Bioengineering
Purifying stem cell-derived red blood cells: a high-throughput label-free downstream processing strategy based on microfluidic spiral inertial separation and membrane filtration.
Authors:Guzniczak E, Otto O, Whyte G, Chandra T, Robertson NA, Willoughby N, Jimenez M, Bridle H.
2020 - Research and Practice in Thrombosis and Haemostasis
Quantifying single-platelet biomechanics: An outsider's guide to biophysical methods and recent advances
Authors:Sachs L., Denker C., Greinacher A., Palankar R.
2020 - Hamostaseologie
Role of Platelet Cytoskeleton in Platelet Biomechanics: Current and Emerging Methodologies and Their Potential Relevance for the Investigation of Inherited Platelet Disorders
Authors:Zaninetti C, Sachs L, Palankar R.
2020 - Biology of the Cell
The mechanics of myeloid cells
Authors:Bashant KR., Toepfner N., Day CJ., Mehta NN., Kaplan MJ., Summers C., Guck J., Chilvers ER.
2020 - Journal of Polymer Science
Tunable polymer microgel particles and their study using microscopy and real‐time deformability cytometry
Authors:Sinjari S, Freitag JS, Herold C, Otto O, Smith DM, Stöver HDH
2019 - Methods in Molecular Biology
Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry
Authors:Jacobi A., Rosendahl P., Kräter M., Urbanska M., Herbig M., Guck J.
2019 - Journal of Cellular and Molecular Medicine
Biallelic CCM3 mutations cause a clonogenic survival advantage and endothelial cell stiffening
Authors:Schwefel K., Spiegler S., Ameling S., Much C.D., Pilz R.A., Otto O., Völker U., Felbor U., Rath M.
2019 - Philosophical Transactions of the Royal Society B: Biological Sciences
Cardiomyocyte mechanodynamics under conditions of actin remodelling
Authors:Pires RH., Shree N., Manu E., Guzniczak E., Otto O.
2019 - IEEE/ACM Transactions on Computational Biology and Bioinformatics
Cell Mechanics Based Computational Classification of Red Blood Cells Via Machine Intelligence Applied to Morpho-Rheological Markers
Authors:Ge Y., Rosendahl P., Duran C., Toepfner N., Ciucci S., Guck J., Cannistraci C.V..
2019 - Stem Cell Research & Therapy
Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis
Authors:Sarem M., Otto O., Tanaka S., Shastri V.P.
2019 - Annals of Hematology
Effects of rigosertib on the osteo-hematopoietic niche in myelodysplastic syndromes
Authors:Balaian E., Weidner H., Wobus M., Baschant U., Jacobi A., Mies A., Bornhäuser M., Guck J., Hofbauer L.C., Rauner M., Platzbecker U.
2019 - Biophysical Journal
High-Throughput Microfluidic Characterization of Erythrocyte Shapes and Mechanical Variability
Authors:Reichel F., Mauer J., Nawaz A.A., Gompper G., Guck J., Fedosov D.A.
2019 - Nature Communications
High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry
Authors:Fregin B., Czerwinski F., Biedenweg D., Girardo S., Gross S., Aurich K., Otto O.
2019 - Cytometry Part A
Morpho-Rheological Fingerprinting of Rod Photoreceptors Using Real-Time Deformability Cytometry
Authors:Santos-Ferreira T., Herbig M., Otto O., Carido M., Karl MO., Michalakis S., Guck J., Ader M.
2019 - Journal of Leukocyte Biology
Real-time deformability cytometry reveals sequential contraction and expansion during neutrophil priming
Authors:Bashant K.R., Vassallo A., Herold C., Berner R., Menschner L., Subburayalu J., Kaplan M.J., Summers C., Guck J., Chilvers E.R., Toepfner N.
2019 - Advanced Science
Spheroid Culture of Mesenchymal Stromal Cells Results in Morphorheological Properties Appropriate for Improved Microcirculation
Authors:Tietze S., Kräter M., Jacobi A., Taubenberger A., Herbig M., Wehner R., Schmitz M., Otto O., List C., Kaya B., Wobus M., Bornhäuser M., Guck J.
2019 - Cancer Research
Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Authors:Surcel A., Schiffhauer E.S., Thomas D.G., Zhu Q., DiNapoli K.T., Herbig M., Otto O., West-Foyle H., Jacobi A., Kräter M., Plak K., Guck J., Jaffee E.M., Iglesias P.A., Anders R.A., Robinson D.N.
2019 - Molecular Biology of the Cell
The relationship between metastatic potential and in vitro mechanical properties of osteosarcoma cells
Authors:Holenstein C.N., Horvath A., Schär B., Schoenenberger A.D., Bollhalder M., Goedecke N., Bartalena G., Otto O., Herbig M., Guck J., Müller D.A., Snedeker J.G., Silvan U.
2018 - Cells
Alterations in Cell Mechanics by Actin Cytoskeletal Changes Correlate with Strain-Specific Rubella Virus Phenotypes for Cell Migration and Induction of Apoptosis
Authors:Kräter M., Sapudom J., Bilz N.C., Pompe T., Guck J., Claus C.
2018 - Molecular Biology of the Cell
Cancer cell ability to mechanically adjust to extracellular matrix stiffness correlates with their invasive potential
Authors:Wullkopf l., West AK. V., Leijnse N., Cox T. R., Madsen C. D., Oddershede L. B., Erler J.T.
2018 - eLife
Detection Of Human Disease Conditions By Single-Cell Morpho-Rheological Phenotyping Of Whole Blood
Authors:Töpfner N., Herold C., Otto O., Rosendahl P., Jacobi A., Kräter M., Stächele J., Menschner L., Herbig M., Ciuffreda L., Ranford-Cartwright L., Grzybek M., Coskun U., Reithuber E., Garriss G., Mellroth P., Henriques-Normark B., Tregay N., Suttorp M., Bornhäuser M., Chilvers E.R., Berner R., Guck J.
2018 - Methods in Cell Biology
High-throughput single-cell mechanical phenotyping with real-time deformability cytometry
Authors:Urbanska M., Rosendahl P., Kräter M., Guck J.
2018 - Biomicrofluidics
Impact of poloxamer 188 (Pluronic F-68) additive on cell mechanical properties, quantification by real-time deformability cytometry
Authors:Guzniczak E., Jimenez M., Irwin M., Otto O., Willoughby N., Bridle H.
2018 - Frontiers in Immunology
Metabolic Profiling of Human Eosinophils
Authors:Porter L., Toepfner N., Bashant K.R., Guck J., Ashcroft M., Farahi N., Chilvers E.R.
2018 - Flow Cytometry Protocols
Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells.
Authors:Herbig M., Kräter M., Plak K., Müller P., Guck J., Otto O.
2018 - Nature Methods
Real-time fluorescence and deformability cytometry
Authors:Rosendahl P., Plak K., Jacobi A., Kraeter M., Toepfner N., Otto O., Herold C., Winzi M., Herbig M., Ge Y., Girardo S., Wagner K., Baum B., Guck J.
2018 - Journal of Materials Chemistry B
Standardized microgel beads as elastic cell mechanical probes
Authors:Girardo S., Träber N., Wagner K., Cojoc G., Herold C., Goswami R., Schlüßler R., Abuhattum S., Taubenberger A., Reichel F., Mokbel D., Herbig M., Schürmann M., Müller P., Heida T., Jacobi A., Ulbricht E., Thiele J., Werner C., Guck J.
2018 - Biomicrofluidics
Statistics for real-time deformability cytometry: Clustering, dimensionality reduction, and significance testing.
Authors:Herbig M., Mietke A., Müller P., Otto O.
2018 - Cell
There Will Be Blood Tests
Authors:Dodgson S.E.
2017 - Nature Communications
Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells
Authors:Tavares S., Vieira AF., Taubenberger AV., Araújo M., Martins NP., Brás-Pereira C., Polónia A., Herbig M., Barreto C., Otto O., Cardoso J., Pereira-Leal JB., Guck J., Paredes J., Janody F.
2017 - Scientific Reports
Bone marrow niche-mimetics modulate HSPC function via integrin signaling.
Authors:Kräter M., Jacobi A., Otto O., Tietze S., Müller K., Poitz DM., Palm S., Zinna VM., Biehain U., Wobus M., Chavakis T., Werner C., Guck J., Bornhäuser M.
2017 - Scientific Reports
High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing
Authors:Guzniczak E., Mohammad Zadeh M., Dempsey F., Jimenez M., Bock H., Whyte G., Willoughby N. & Bridle H.
2017 - Cytoskeleton
High-throughput cell mechanical phenotyping for label-free titration assays of cytoskeletal modifications.
Authors:Golfier S., Rosendahl P., Mietke A., Herbig M., Guck J., Otto O.
2017 - Blood
Initiation of acute graft-versus-host disease by angiogenesis
Authors:Riesner K, Shi Y, Jacobi A, Kraeter M, Kalupa M, McGearey A, Mertlitz S, Cordes S, Schrezenmeier JF, Mengwasser J, Westphal S, Perez-Hernandez D, Schmitt C, Dittmar G, Guck J, Penack O.
2017 - arXiv.org
Mapping of Deformation to Apparent Young's Modulus in Real-Time Deformability Cytometry
Authors:Herold C.
2017 - ACS Biomaterials Science & Engineering
Numerical Simulation of Real-Time Deformability Cytometry To Extract Cell Mechanical Properties
Authors:Mokbel M., Mokbel D., Mietke A., Träber N., Girardo S., Otto O., Guck J., and Aland S.
2017 - Proceedings of the National Academy of Sciences
Plasmodium falciparum erythrocyte-binding antigen 175 triggers a biophysical change in the red blood cell that facilitates invasion.
Authors:Koch M., Wright KE., Otto O., Herbig M., Salinas ND., Tolia NH., Satchwell TJ., Guck J., Brooks NJ., Baum J.
2017 - Development
Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage
Authors:Urbanska M., Winzi M., Neumann K., Abuhattum S., Rosendahl P., Müller P., Taubenberger A., Anastassiadis K., Guck J.
2017 - Oxidative Medicine and Cellular Longevity
Toxicity and Immunogenicity in Murine Melanoma following Exposure to Physical Plasma-Derived Oxidants
Authors:Bekeschus S., Rödder K., Fregin B., Otto O., Lippert M., Weltmann KD., Wende K., Schmidt A., Gandhirajan RK.
2017 - Oncotarget
V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling - a new option for HCC therapy
Authors:Bartel K., Winzi M., Ulrich M., Koeberle A., Menche D., Werz O., Müller R., Guck J., Vollmar A.M, von Schwarzenberg K.
2016 - eLife
A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy
Authors:M. C. Munder, D. Midtvedt, T. Franzmann, E. Nüske, O. Otto, M. Herbig, E. Ulbricht, P. Müller, A. Taubenberger, S. Maharana, L. Malinovska, D. Richter, J. Guck, V. Zaburdaev, S. Alberti.
2016 - Integrative Biology
Mechanical phenotyping of primary human skeletal stem cells in heterogeneous populations by real-time deformability cytometry
Authors:M. Xavier, P. Rosendahl, M. Herbig, M. Kräter, D. Spencer, M. Bornhäuser, R. O. C. Oreffo, H. Morgan, J. Guck, O. Otto.
2016 - Molecular Metabolism
The F-actin modifier villin regulates insulin granule dynamics and exocytosis downstream of islet cell autoantigen 512
Authors:Mziaut H, Mulligan B, Hoboth P, Otto O, Ivanova A, Herbig M, Schumann D, Hildebrandt T, Dehghany J, Sönmez A, Münster C, Meyer-Hermann M, Guck J, Kalaidzidis Y, Solimena M.
2015 - Oncotarget
Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia
Authors:M. Wobus, M. Bornhäuser, J. Guck, O. Otto, A. Jacobi, M. Kräter, C. Ortlepp, G. Ehninger, Ch. Thiede, U. Oelschlägel
2015 - Biophysical Journal
Cell Mechanics: Combining Speed with Precision
Authors:Wyss H.M.
2015 - Biophysical Journal
Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment
Authors:A. Mietke, O. Otto, S. Girardo, P. Rosendahl, A. Taubenberger, S. Golfier, E. Ulbricht, S. Aaland, J. Guck, E. Fischer-Friedrich
2015 - Biophysical Journal
Myosin II Activity Softens Cells in Suspension
Authors:C. J. Chan, A. E. Ekpenyong, S. Golfier, W. Li, K. J. Chalut, O. Otto, J. Elgeti, J. Guck, F. Lautenschläger
2015 - Nature Methods
Real-time deformability cytometry: on-the-fly cell mechanical phenotyping
Authors:O. Otto, Ph. Rosendahl, A. Mietke, S. Golfier, Ch. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jacobi, M. Wobus, N. Töpfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, J. Guck
2013 - Science Translational Medicine
Mechanics Meets Medicine
Authors:Guck J., Chilvers E.R.
