Cell characterization with light and force

The AcCellerator is your door to the world of cellular mechanics. Extend your research methods with a quantitative cell characterization device that detects properties, responses and function that would otherwise remain hidden.

The AcCellerator is a high speed imaging cytometer for mechanical cell characterization through real-time deformability cytometry measurements. Designed as an extension to an inverted microscope, the combination of hardware and software solutions ensures unprecedented comfort and flexibility for cell mechanical measurements. Our InitialService includes delivery, installation and user training as well as all the consumables necessary for your first experiments.

the accellerator:
A combination of fast imaging, flow cytometry and force application for single cells.

Force

Explore physical properties of cells as a biomarker. Using the AcCellerator   , you can apply non-destructive forces to cells or beads and observe their deformation. This allows to study the specific mechanical response  to physical stress.

  • mechanics as a new biomarker
  • label-free
  • non-destructive forces

Imaging

Every cell is photographed, analyzed and stored simultaneously. This allows to find small subpopulations or distinguish cells by  means of  their optical properties.  Additionally one  can study morphological properties like surface topology or the attenuation of light by the cell.

  • storage of every acquired image
  • quick access to cell size and morphology


Cytometry

Parameters like deformation, brighness and size are extracted while the cell is passing the channel. This allows to gate for cell properties in real-time.

  • up to 1000 cells per second
  • no cell isolation/purification necessary

Do you want to combine real-time deformability cytometry and simultaneous fluorescence detection ? Check out the FluorescenceModule  !


accellerator applications

Detect changes of the cytoskeleton:

Alterations of the cytoskeleton can be quantified through mechanical analysis. The depletion of actin microfilaments by Cytochalasin D results in a higher deformation and therefore, reduced stiffness of HL60 cells. The plot below shows the superposition of treated and untreated cells.

Investigate effects of past conditions:

Primary human hematopoietic stem cells (HSCs), are commonly identified by the presence of the transmembrane protein CD34. The plot below compares CD34+ cells obtained from bone marrow and CD34+ cells that were mobilized into peripheral blood by the granulocyte colony–stimulating factor (G-CSF). While identical according to their CD34+ classification, HSCs derived from peripheral blood are stiffer than HSCs derived from bone marrow.

Discern blood cell types:

Many cell types are distinguishable by properties of their images like brightness and size. In whole blood samples, this enables the identification and further analysis of erythrocytes (red blood cells), thrombocytes (platelets) and even subpopulations of leukocytes (white blood cells) without the need of labeling or cell purification.

Resolve kinetics in neutrophil activation:

High measurement rates and fast sample preparation allow for observation of kinetic processes. The plot below shows the change of mechanical properties when neutrophil granulocytes from freshly drawn blood are exposed to formyl-methionyl-leucyl-phenylalanin (fMLP). The tripeptide fMLP is released by many bacteria and signals an infection to cells of the immune system.

software for data aquisition

Shape-In

Shape-In allows you to define your measurement parameters and to control measurement conditions. The created data file contains information on all detected events including image data, deformation data and morphology data. Real-time gating allows you to focus on a subpopulation of cells within a heterogeneous sample.

Software for data analysis: click here  shape-out

The all-inclusive package includes:

  • Inverted microscope Zeiss AxioObserver  with xy stage and 40x/0.65 microscopy objective  (options: manual or motorized)
  • High-speed CMOS camera
  • High-power LED illumination  for microsecond stroboscopic  flashes triggered by the camera
  • High-precision syringe pump with two syringe modules
  • Sample holder with magnetic fixation, rotation platform  and cover box with fast clamping for the tubing
  • Dedicated computer, ready-to-use, including frame grabber (options: Windows-PC or -Laptop)
  • Preconfigurated measurement software Shape-In2
  • Free open source analysis software Shape-Out    for post-experimental data evaluation
  • Delivery, installation, teaching of the users
  • Free software updates for one year
  • Consumables for the first 100 experiments

The price

The AcCellerator  starts from 114 000 €. The price depends on your needs for a microscope or customizations and includes everything from shipping, to installation, to teaching and consumables for the first 100 experiments. All you need are 2 m² on a stable table in temperature stable room. 

(only for business customers, excl. VAT)

Download a pdf-flyer of all our products, including the specifications. click here!

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